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KMID : 0984920150170020119
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Journal of Skin Barrier Research
2015 Volume.17 No. 2 p.119 ~ p.119
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Regulation of IL-1α, IL-6 and Human β Defensin-2 in Human Epidermal Keratinocytes Exposed to Staphylococcus Aureus by Epidermal Growth Factor
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Kim Ji-Min
Choo Jung-Eun
Min Kyung-Hyun
Chang Sung-Eun
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Abstract
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Staphylococcus aureus (S. aureus) skin colonization is universal in atopic dermatitis (AD) which is characterized by impaired skin barrier and decreased antimicrobial peptides (AMPs) with defective innate immune function. S. aureus also is known to increase in the patients who get epidermal growth factor (EGF) receptor inhibitors for the treatment of various cancers. Despite EGF has been successfully used topically to promote wound healing, potential role of EGF in chronic skin inflammatory disorders including AD has not been clarified. Thus, we investigated the effect of EGF on inflammatory cytokines and AMPs by ELISA assay in human epidermal keratinocyte treated with heat-inactivated S. aureus (HKSA). Intracellular signaling pathways such as ERK, NFκB and p38 were investigated using quantitative PCR. HKSA-induced IL-6 expression and mRNA levels of NFκB and p38 were decreased by EGF. Intriguingly, EGF increased the expression of IL-1α and human β defensin 2 (hBD2). Taken together, EGF may contribute to relieve inflammatory signals induced by S. aureus and to improve skin barrier function, suggesting a possible role of EGF in AD.
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KEYWORD
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EGF, S. aureus, Inflammatory cytokines, hBD2, Skin Barrier
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